Siroz hastalarında alkalen fosfataz izoenzimlerinin iki farklı yöntemle belirlenmesi


Thesis Type: Postgraduate

Institution Of The Thesis: Sivas Cumhuriyet University, Tıp Eğitimi, Temel Tıp Bilimleri Bölümü, Turkey

Approval Date: 2008

Thesis Language: Turkish

Student: SERHAT AKÇA

Consultant: Veysel Kenan Çelik

Abstract:

Alkaline phosphatases is glycoprotein structured metalophosphatase with several defined functions. The enzyme may catalyse the hydrolysis of various monophosphate esters at alkaline pH. İt is present in many tissues of all living beings from bacteria to mammals. The greatest concentrations of ALP are found in bone, liver, intestine, and the placenta.To understand which tissue caused the ALP increase specific tissue isoenzymes of ALP must be definedThe aim of this study is; to determine the ALP isoenzymes in 100 serum samples, which are obtained from 50 controls and 50 cirrhosis patients, via heat inactivation and agarose gel electrophoresis methodsValues of ALP liver isoenzyme abtained by Agarose gel electrophoresis method (Gel LALP) and values of ALP liver isoenzyme obtained by Heat inaktivation method (Heat LALP) were compared with each other either as U/L value or as a percentage of total ALP value in both control and patient group and the results were evaluated statisticallyHeat LALP values (U/L) were 25.08 ± 7.75 for control group, 37.00 ± 14.58 for patient group. Gel LALP values were 39.24 ± 15.67 for control group, 51.83 ± 21.18 for patient group. Difference between mean values were found to be statistically significant (p<0.05Heat LALP values (percentage for activity) were 38.93 ± 7.83 for control group, 40.56 ± 9.38 for patient group. Gel LALP values were 59.81 ± 14.20 for control group, 58.19 ± 16.80 for patient group. Difference between mean values were found to be statistically significant (p<0.05As a result, heat inactivation method performed at 56 degree for 10 minutes was found not to be sensitive enough in determination of isoenzymes which causes increase in serum total ALP activity.Key Words: Alkaline phosphatase, cirrhosis, isoenzymes, agarose gel electrophoresis, heat inactivation