Pseudomonas putida NRRL B-13 suşu tarafından ksilenin biyolojik yıkımının optimizasyonu

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Thesis Type: Postgraduate

Institution Of The Thesis: Sivas Cumhuriyet University, Fen Fakültesi, Biyoloji Bölümü, Turkey

Approval Date: 2019

Thesis Language: Turkish

Student: SADİ SEZGİNER

Supervisor: Musa Sari

Open Archive Collection: AVESIS Open Access Collection

Abstract:

ABSTRACT OPTİMİZATİON OF BİOLOGİCAL DEGRADATİON OF XYLENE BY PSEUDOMONAS PUTİDA STRAİN NRRL B-13 Sadi SEZGİNER Master of Science Thesis, Department of Biology Supervisor: Doç. Dr. Musa SARİ 2019; xv + 34 page Petroleum and its derivatives are used in almost all of our daily lives and are indispensable in our lives. The types of waste generated in this production chain, the environmental impacts of the production processes and the disposal methods of production wastes were evaluated, the recycling practices of the wastes generated after the use of the products were examined and the necessity of recycling was discussed. The aim of this study is to provide biodegradation of xylene which is one of the industrial aromatic pollutants and degraded by Pseudomonas putida NRRLB-13 which is one of the microorganisms that perform biological degradation of xylene. Pseudomonas putida strains NRRL B-13 were activated to proliferate in the medium containing glucose for two generations. 4 mM of xylene was added to 250 ml of medium and the media were autoclaved. The autoclaved medium (250 ml) was inoculated with 4 ml of 2nd generation bacteria. Absorbance measurement was performed on the spectrophotometer every 4 hours at 600 nm wavelength by taking 2 ml sample from the medium from the first time, a gradual increase in the absorbance values was observed, which shows us that bacteria produce and xylene is used as energy source. Xylene absorbance values were measured every 8 hours at 260 nm in bacteria culture medium containing 8 mM xylene and 96 hours time period. Xylene absorbance values decreased gradually. This clearly demonstrated that Pseudomonas putida bacteria were using xylene. When the absorbance values of the 8 mM xylene-containing bacteria culture at 600 nm 30 oC and time-dependent change values were examined, it was seen that the decrease in temperature negatively affected the bacterial growth. When the absorbance values of MgCl2-containing and non-MgCl2-containing media are analyzed, a faster decrease in absorbance in MgCl2-containing media indicates that MgCl2 is effective in xylene degradation. CaCl2 has no effect on xylene degradation. It is thought that CaCl2 increases the absorbance in the medium containing CaCl2. Key Words: Pseudomonas sp. xylene, biodegradation.