Vitamin B2 (Riboflavin)'in Pratik ve Hassas Analizi için Analitik Yöntemin Geliştirilmesi

Abstract

Riboflavin (vitamin B2) is one of the essential water-soluble vitamins and has an increasing use both in the natural biological environment and in food additives and other pharmaceutical areas. The accurate and precise determination of the amount of this vitamin is of great importance in terms of food safety, human health and pharmacological applications. Riboflavin is a fundamental element in understanding the relationship between nutrition and health, as it acts as a coenzyme in metabolic processes and plays a critical role in vital functions such as energy production and antioxidant defense mechanisms. Riboflavin deficiency can lead to various health problems, especially ariboflavinosis. These problems include symptoms such as cracks in the skin, sores at the corners of the mouth, inflammation of the tongue, light sensitivity, blurred vision, corneal inflammation, weakness, and impaired nervous system functions. Therefore, early diagnosis and treatment of deficiency is of great importance in terms of public health and clinical applications. In addition, accurate analysis of riboflavin content is a critical step in areas such as the formulation of food supplements, quality control of pharmaceutical products and optimization of nutritional programs. Therefore, both accurate and sensitive analysis of riboflavin stands out as a great necessity in terms of both health and industrial applications. In the proposed project study, an analytical approach including spectrophotometric determination method after solid phase extraction will be developed in order to analyze vitamin B2 in real samples. The analysis of target molecules separated and enriched by the applied solid phase extraction will be done by spectroscopic method at appropriate wavelength and analytical validation of the developed method will be done according to international guidelines. Finally, the developed method will be applied to food supplements and vitamin support drugs after being verified with model solutions.