Akademik Veri Yönetim Sistemi
2ST cancer days, Sivas, Türkiye, 16 - 19 Eylül 2021, cilt.1
The use of bee pollen, which is a natural honey-bee product, as a healthy nutritional supplement by
consumers has become very common nowadays [1-3]. The chemical composition of bee products varies greatly
according to the geographical structure of the area where they are collected, climatic conditions and floral
richness. The broad-spectrum biological activity values of bee products can also vary according to the
differences in their content. In this case, it means that the therapeutic activities of bee products obtained from
different regions may vary. In short, the chemical composition of bee products will include the chemical
components of the floral structure in the region where they are obtained. Studies in the literature have shown
that there are significant differences between total phenolic contents and antioxidant activities of bee pollen
from different botanical origins and different regions [4-5]. In this study, it was aimed to extract bee pollen
samples collected from different regions of Sivas province by using 2 different solvents and to compare their
in vitro cytotoxic activities using the MCF-7 (Human breast cancer) cell line. In general, it will be possible to
compare the effectiveness of Sivas samples with the studies in the literature. Extraction of pollen samples
obtained from Yıldızeli, Zara and center districts of Sivas province in the 2020 harvest period was carried out
using ethyl alcohol and water solvents. The biological activities of the isolates were investigated in vitro on the
MCF-7 cell line using the MTT cell proliferation kit. Using the MTT results, IC50 values were calculated in
the Graphpad analysis program. It was observed that pollen isolates purified using 2 different solvents showed
good cytotoxic activity against MCF-7 cell line. It was determined that ethyl alcohol isolates of pollen samples
showed IC50 values in the range of 5.0-68.8 μg/mL and water isolates 45.5-86.5 μg/mL, and ethyl alcohol
isolates of pollen samples generally showed higher activity than water isolates. It is thought that the pollen
samples collected from various localities in the province of Sivas and extracted with ethyl alcohol and water
under laboratory conditions are promising in the preparation of new and effective pharmacognostic
supplements.
Keywords: Antiproliferative activity, bee products, MCF-7, pollen, Sivas