Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors

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TAŞ A., Gumus E., Ozmen E., ERDOĞAN H., SİLİĞ Y.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.46, ss.709-719, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1515/tjb-2021-0160
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.709-719
  • Anahtar Kelimeler: BAP1, basal cell carcinoma, expression, eyelid, OGT, tumor, YY1, YIN YANG 1, HYPER-O-GLCNACYLATION, UVEAL MELANOMA, MUTATIONS PREDISPOSE, CELL-PROLIFERATION, CANCER, CARCINOMA, TUMORIGENESIS, METABOLISM, TRANSITION
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Objectives The aim of this study was to investigate BAP1, OGT and YY1 genes and protein levels in 12 samples (8 males, 4 females) of eyelid tumor tissue with basal cell carcinoma (BCC) and 12 normal control subjects (8 males, 4 females). Methods The expression levels of these genes were determined with RT-PCR and the protein levels and expression using ELISA and IHC methods, respectively. Results In RT-PCR analysis, statistically significant upregulated expression was determined of 1.84-fold of BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05). In the patient group, compared to the control group, there was a similar statistically significant strong correlation between the proteins (BAP1 and YY1; r = 0.850, BAP1 and OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the ELISA and IHC analysis methods, a significant increase in BAP1 and YY1 protein expression levels was observed compared to the control group (p < 0.05). Conclusions The study results demonstrated that BAP1 and YY1 genes and protein levels were upregulated in eyelid tumor tissue with BCC.