Optimization of a rapid and sensitive ultrasound-assisted liquid-liquid microextraction using switchable hydrophilicity solvent for extraction of β-carotene in fruit juices and vegetables

ALTUNAY N., Tuzen M., Lanjwani M. F., Mogaddam M. R. A.

Journal of Food Composition and Analysis, cilt.114, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 114
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.jfca.2022.104791
  • Dergi Adı: Journal of Food Composition and Analysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: beta-carotene, Real samples, Microextraction, Switchable, Spectrophotometer, Factorial design
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

© 2022 Elsevier Inc.A simple, rapid and sensitive ultrasound-assisted liquid-liquid microextraction by switchable hydrophilicity solvent (UA-LLME-SHS) method was developed first time for extraction and determination of β-carotene from fruit juices and vegetables using a spectrophotometer. The effect of pH, solvent dose, ultrasound time, volume of NH4OH, volume of NaCl (w/v) and effects of different chemicals on extraction recovery was investigated. The proposed method showed good extraction recovery of β-carotene. The working range of the developed method was 50–700 ng mL−1. Limits of detection and quantification were 15.5 μg L-1 and 50 μg L-1, respectively. The intraday and interday precisions of the developed method was investigated for 75 ng mL−1 and 400 ng mL−1 of β-carotene. As a result of the study, the relative standard deviation values were in the range of 2.8–3.3 % and 3.0–4.2 % for the intraday and interday studies, respectively. The enhancement factor was 114 and extraction recovery of β-carotene was found 98.3 %. The factorial design was drawn to know the combined effects of variables and their significant and insignificant effects on the determination of the β-carotene.