Induction of ovulation using repeated doses of sulpiride, a dopamine antagonist, in ewe lambs


Kıvrak M. B., Aydin I.

Reproduction in Domestic Animals, cilt.58, sa.3, ss.379-386, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 3
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1111/rda.14295
  • Dergi Adı: Reproduction in Domestic Animals
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.379-386
  • Anahtar Kelimeler: dopamine antagonist, ewe lamb, FSH, LH, LUTEINIZING-HORMONE SECRETION, FOLLICLE-STIMULATING-HORMONE, PULSATILE LH-SECRETION, NEGATIVE FEEDBACK, PULSE FREQUENCY, PUBERTY, GNRH, SUPPRESSION, ESTRADIOL, MELATONIN
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

© 2022 Wiley-VCH GmbH.This study aimed to test the hypothesis that sulpiride can increase the concentration of circulating gonadotropin that can promote puberty in pre-pubertal ewe lambs. Here, 12 1–3-year-old Merino rams and 60 7–9-month-old Merino sheep were included in the study. The sheep were randomly divided into sulpiride (n = 30) and control (n = 30) groups. The sulpiride group was subcutaneously injected with 0.6 mg/kg sulpiride twice daily (morning and evening) for 9 days. During these 9 days, blood samples were taken from the sheep before drug administration and at 4 h after every drug administration. The number of ovulating animals in the sulpiride group was significantly higher than that in the control group (90% vs. 32%). No oestrous signs were observed in either group during ram release. Further, there were no differences in the levels of mean follicle-stimulating hormone in the two groups based on treatment (p =.2), time (p =.3) or treatment-by-time interaction (p =.3). After sulpiride administration, the luteinizing hormone (LH) levels of the sulpiride group rapidly increased and remained stable for a long time, whereas physiological LH fluctuations in the control group remained unchanged. Within-group changes in terms of LH concentrations were significant for both groups (p <.001), whereas LH pulse frequency was significantly different between the sulpiride group (p =.03). Therefore, it is concluded that sulpiride can be used as a non-steroidal alternative to stimulate pre-pubertal ewe lambs and sheep during anoestrus.