In this study, it was aimed to determine of diagnostic importance of blood lipid levels in neonatal calves with sepsis. The study was carried out on a total of 70 calves, 60 with sepsis and 10 healthy calves. The calves with sepsis were included in the study, according to clinical and hematological findings. The blood samples were taken from the V. jugularis for hematological, lipid profile and biochemical analyzes after the routine clinical examinations of the calves. There were significantly (P < 0.05) decrease in body temperature, increase in respiration rate and capillary refill time in the calves with sepsis compared to control group. The levels of blood urea nitrogen, creatinine concentrations of calves with sepsis were significantly higher (P < 0.05), however, levels of total cholesterol, HDL and LDL concentrations were significantly lower (P < 0.05) than control group. In addition, blood triglyceride and VLDL concentrations of calves with sepsis were higher than control group, however there was no statistical difference.