Research on Hotair and 7SL-RNA Gene Expression Levels in Psoriasis Vulgaris


Yazici S., Guner R., AKYOL M., Bayyurt E. B. , Arslan S.

INDIAN JOURNAL OF DERMATOLOGY, vol.66, no.6, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 66 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.4103/ijd.ijd_1125_20
  • Journal Name: INDIAN JOURNAL OF DERMATOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: 7SL-RNA, HOTAIR, non-coding genes, psoriasis, LONG NONCODING RNAS, 7SL RNA
  • Sivas Cumhuriyet University Affiliated: Yes

Abstract

Backgrounds: Mutation of protein-coding genes and non-coding genes is a factor in psoriasis etiology. Non-coding RNA (ncRNA), which does not have protein-coding capacity, is available in the human genome. HOTAIR (HOX Antisense Intergenic RNA) and 7SL-RNA are known as ncRNA. They may play a role in psoriasis pathogenesis. Aims: In our study, we aimed to investigate the level of HOTAIR and 7SL-RNA gene expression in the lesional and perilesional healthy skin of psoriasis patients. Methods: Total RNA isolation from the skin samples was achieved by modifying the RNeasy Mini Kit (Qiagen, Cat No: 74104) protocol. Real Time Polymerase Chain Reaction (qPCR) phase was performed in accordance with the protocol of the relevant brand (WizPure qPCR). Results: 7SL-RNA gene expression decreased in the skin with psoriatic lesions (FC: 0.01; p: 0.028), and this decrease was statistically significant. HOTAIR gene expression decreased (FC: 0.92; p: 0.218), but this decrease was not statistically significant. Conclusions: lncRNAs may play a role in the pathogenesis of psoriasis disease.