A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.

Eroglu C., Yildiz E., Ozturk M., Pinarbasi E.

Acta virologica, vol.44, no.1, pp.29-33, 2000 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 1
  • Publication Date: 2000
  • Title of Journal : Acta virologica
  • Page Numbers: pp.29-33


In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned sequenced expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.