Circular dichroism spectroscopic investigation of double-decker phthalocyanine with G-Quadruplex as promising telomerase inhibitor


BAĞDA E., BAĞDA E., YABAŞ E.

JOURNAL OF MOLECULAR STRUCTURE, vol.1127, pp.716-721, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 1127
  • Publication Date: 2017
  • Doi Number: 10.1016/j.molstruc.2016.07.093
  • Journal Name: JOURNAL OF MOLECULAR STRUCTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.716-721
  • Sivas Cumhuriyet University Affiliated: Yes

Abstract

In the present study, interaction of a double-decker phthalocyanine with two G-quadruplex DNA, Tel 21 and cMYC, was investigated. To the best of our knowledge, this is the first study about G-quadruplex-double decker phthalocyanine interaction. The spectrophotometric titration method was used for binding constant calculations. From the binding constants, it can be said that double-decker phthalocyanine more likely to bind Tel 21 rather than cMYC. The conformational changes upon binding were monitored via circular dichroism spectroscopy. The ethidium bromide replacement assay was investigated spectrofluorometrically. (C) 2016 Published by Elsevier B.V.