Effect of Pre-Treatment with Acetaminophen on Hippocampal Oxidative, Inflammatory, and Apoptotic Parameters in PTZ-Induced Acute Seizure Mice Model


KARABULUT S., TAŞKIRAN A. Ş.

NEUROCHEMICAL JOURNAL, vol.15, no.1, pp.79-85, 2021 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.1134/s1819712421010050
  • Journal Name: NEUROCHEMICAL JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.79-85
  • Keywords: neuroinflamattion, oxidative stress, PTZ, acetaminophen, NITRIC-OXIDE, ACID
  • Sivas Cumhuriyet University Affiliated: Yes

Abstract

The current investigation aimed to determine the effects of pre-treatment of acetaminophen on seizure susceptibility and expressions of hippocampal oxidative-nitrosative stress and inflammatory markers after exposure to pentylenetetrazole administration in a mouse model. The seizure symptoms of pentylenetetrazole-treated mice were assessed using Racine classification and latency to first myoclonic jerk. Then, several parameters including TAS, TOS, NO, TNF-alpha, caspase-3, GABA, and glutamate levels were quantitated by commercial kits. The pre-treatment of acetaminophen significantly suppressed pentylenetetrazole-induced seizures. Also, it significantly increased the TAS and GABA values and significantly decreased the TOS, NO, TNF-alpha, caspase-3, and glutamate values in the hippocampus. These findings are indicative of the antiseizure potential of pre-treatment with acetaminophen in the pentylenetetrazole-induced acute seizure model of epilepsy. This effect may be due to its impact on antioxidant, anti-inflammatory, and anti-apoptotic pathways.