Optimization of ultrasound-assisted dispersive liquid-liquid microextraction of niacinamide in pharmaceutical and cosmetic samples using experimental design

ALTUNAY N.

MICROCHEMICAL JOURNAL, cilt.170, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.microc.2021.106659
  • Dergi Adı: MICROCHEMICAL JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Anahtar Kelimeler: Box-Behnken design, Alcohol-based deep eutectic solvent, Niacinamide, Optimization, Dispersive liquid-liquid microextraction, Pharmaceuticals, DEEP EUTECTIC SOLVENTS, CLOUD-POINT EXTRACTION, WATER-SOLUBLE VITAMINS, NICOTINAMIDE, ACID, SPECTROPHOTOMETRY, SEPARATION, B-3, B3
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this paper, an efficient and inexpensive analytical procedure using sugar alcohol-based deep eutectic solvent ultrasound-assisted dispersive liquid-liquid microextraction (DES-UA-DLLME) was optimized for the extraction of niacinamide from vitamin supplements, syrups and cosmetic samples. After the DES-UA-DLLME procedure, the niacinamide content in the samples was determined by UV-VIS spectrophotometer at 265 nm. Box-Behnken design was applied for the optimization of important variables that have a positive effect on extraction recovery of niacinamide. The optimum conditions included: DES-4 solution (mixture of sorbitol and glycerol with a molar ratio of 2:1) volume, 335 mu L; pH, 6.1; sonication time 1.5 min; and extraction temperature, 30 degrees C. Using the optimum values, the DES-UA-DLLME procedure provides good linear range (1-400 ng mL(-1)), low detection limit (0.33 ng mL(-1)), high enrichment factor (227), quantitative recovery (97.3-98.9%) and good precision (1.4-2.8%). To investigate the validity of the optimized methodology, two standard reference material were analyzed with the DES-UA-DLLME-UV-VIS method. No significant difference was observed between the measured values and the reference values. The DES-UA-DLLME procedure can be successfully applied in routine analysis for extraction and determination of niacinamide in vitamin supplements, syrups and cosmetic samples.