According to the data obtained in this study, Thymus pectinatus has antioxidant and enzyme inhibitory activities while has not showed antimicrobial activities. The results of the current study provide valuable information, showing that the major component of the water extract of T. pectinatus is "1,3-Propanediol, 2 -methyl-, dipropanoate" (59.34 %). This is the first investigation of the antioxidant and enzyme inhibitory activity of T. pectinatus. The results showed that the water extract had high antioxidant, anti-amylase, and anticancer effects, and a higher total phenolic content. Therefore, further phytochemical and bioactivity-guided isolation of T. pectinatus water extract could be carried out to identify the active compounds.