Microsomal epoxide hydrolase polymorphisms


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PINARBAŞI H. , SİLİĞ Y. , PINARBAŞI E.

MOLECULAR MEDICINE REPORTS, vol.3, no.4, pp.723-727, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 3 Issue: 4
  • Publication Date: 2010
  • Doi Number: 10.3892/mmr_00000324
  • Title of Journal : MOLECULAR MEDICINE REPORTS
  • Page Numbers: pp.723-727
  • Keywords: microsomal epoxide hydrolase, polymorphism, GLUTATHIONE-S-TRANSFERASE, OBSTRUCTIVE PULMONARY-DISEASE, LUNG-CANCER RISK, GENETIC POLYMORPHISMS, CIGARETTE-SMOKING, ARACHIDONIC-ACID, SUSCEPTIBILITY, DNA, POPULATION, METABOLISM

Abstract

Microsomal epoxide hydrolase plays a dual role in the activation and detoxification of carcinogenic compounds. Two polymorphic sites have been described in exons 3 and 4 of the microsomal epoxide hydrolase gene that change tyrosine residue 113 to histidine (Tyr113His) and histidine 139 to arginine (His139Arg), respectively. The exon 3 polymorphism reduces enzyme activity by approximately 50%, whereas the exon 4 polymorphism causes a 25% increase in activity. In the present study, the distribution of these polymorphisms in a Turkish population including 625 unrelated healthy individuals was examined using a PCR-RFLP method. The observed genotype frequencies of microsomal epoxide hydrolase exon 3 were 54,38 and 8% for Tyr113Tyr, Tyr113His and His113His, respectively. Exon 4 genotype frequencies were found to be 69, 29 and 2% for His139His, His139Arg and Arg139Arg, respectively.