• Ana Sayfa

Deri Leishmaniosis’i Etkenleri Üzerine, Endemik ve Endemik Olmayan Bitkilerin (Özütlerinin) Antileishmanial Etkilerinin Araştırılması

Doç. Dr. AHMET DURAN ATAŞ

Tez Türü: Doktora

Tezin Yürütüldüğü Kurum: Sivas Cumhuriyet Üniversitesi, Sağlık Bilimleri Enstitüsü, TIP FAKÜLTESİ- TIBBİ PARAZİTOLOJİ AD., Türkiye

Tez Danışmanı: Semra Özçelik

Tezin Onay Tarihi: 2002

Tezin Dili: Türkçe

Desteklendiği Program: Diğer

Özet:

SUMMARY

            The present work was designed to study the in vitro susceptibility of exctracts of 30 plants in order to determine more effective alternatives to antileishmanial drugs which currently being used for treatment.

            Eight different Leishmania tropica strains were used in this study and they were collected from the region of Adana and Şanlıurfa. The inhibitor effects of plant exctracts on proliferation of L. tropica promastigotes on day fourth was evaluated by using of haemocytometer tecnique.

            There was no significant different effects of plant exctracts on activities among L. tropica strains.

            Thirty methil alcohol exctracts which obtained from 26 different plants that were harvested in the region of Sivas, were studied against to 8 different L. tropica strains. Sixteen of those exctracts in the IC50 concentrations were found that they have an antileishmanial activity compared to  Glucantime®.  Glucantime® IC50 value was found as 2 mg/ml when it was compared to same concentration of plant excracts.

            In this study, 26 plants were studied in the 6 different family and the most effectives strains were as follow: Allium scorodoprasum ssp. rotundum (IC50 0,1 mg/ml), Allium nevsehirense –bulb (IC50 0,3 mg/ml), Tanacetum balsamita ssp. balsamita (IC50 0,4 mg/ml),  Tanacetum parthenium –flowers (IC50 0,4 mg/ml), Pimpinella anicetum (IC50 0,4 mg/ml),  Tanacetum densum  ssp. amani (IC50 0,4 mg/ml),  Origanum acutidens (IC50 0,4 mg/ml), Thymus pectinatus var. pectinatus (IC50 0,5 mg/ml), Tanacetum densum  ssp. sivasicum (IC50 0,7 mg/ml), Tanacetum parthenium –aqueous and chloroform parts (IC50 0,7 mg/ml), Allium sivasicum -bulb (IC50 0,9 mg/ml), Salvia ruselli (IC50 0,9 mg/ml), Allium nevsehirense -aerial (IC50 1 mg/ml), Allium sivasicum -aerial (IC50 1 mg/ml), Salvia cryptantha (IC50 1 mg/ml),  Allium dictyoprasum (IC50 2 mg/ml), respectively.

Those plants did not show antileihmanial activity when they were compared to Glucantime® were as follow: Salvia virgata (IC50 2 mg/ml), Achillea teretifolia (IC50 3 mg/ml), Salvia candidissima (IC50 3 mg/ml), Helichrysum chionophylum - aqueous and chloroform parts (IC50 3 mg/ml), Helichrysum arenarum ssp. aucheri (IC50 4 mg/ml), Helichrysum chionophylum -flowers (IC50 4 mg/ml), Astragalus densifolius (IC50 5 mg/ml), Helichrysum plicatum ssp. plicatum (IC50 5 mg/ml), Pelargonium endlicherianum (IC50 5 mg/ml), Astragalus melanophourius (IC50 6 mg/ml), Thymus sipyleus ssp. sipyleus (IC50 6 mg/ml), Helichrysum noeanum (IC50 6 mg/ml), Thymus sipyleus ssp. rosulens (IC50 7 mg/ml), Salvia syriaca (IC50 7 mg/ml).