Effect of the Physalis peruviana and Linum usitatissimum Extracts Against Toluene-Induced Oxidative Damages in Kidney and Liver Tissues of Rats


Gökçe Z., Yılmaz Ö., Zengin H.

Current Perspectives on Medicinal and Aromatic Plants, vol.4, no.1, pp.36-49, 2021 (Other Refereed National Journals)

  • Publication Type: Article / Article
  • Volume: 4 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.38093/cupmap.900518
  • Title of Journal : Current Perspectives on Medicinal and Aromatic Plants
  • Page Numbers: pp.36-49

Abstract

Abstract Toluene causes a great environmental problem, because it is widely used in the industry. This study was intended to examine the effect of golden berry (Physalis peruviana, PP) and flaxseed (Linum usitatissimum, LU) extracts on the malondialdehyde (MDA), reduced glutathione (GSH) fatty acid and lipophilic vitamin content in the kidney and liver tissues of Sprague Dawley male rats, which were oxidative stressed by toluene. PP and LU were extracted and then subjected to vitamin and flavonoid analyses. The rats were divided into four groups as control (C), toluene (T), toluene + P. peruviana (T + PP), and toluene + L. usitatissimum (T + LU). T was injected intraperitoneally at a concentration of 0.3 mL/kg. In addition, PP and LU were also injected intraperitoneally at a concentration of 0.5 mL/kg. The treatments were maintained for two months. Afterwards, the rats were decapitated and tissues were removed. Tissue samples were homogenized using buffer solutions. MDA, GSH, lipophilic vitamin, cholesterol and fatty acid content was also determined. In conclusion, MDA concentration level significantly increased and GSH concentration level significantly decreased in the toluene group (p<0.001; p<0.001). GSH level increased and the MDA concentration decreased in T+PP and T+LU treated groups (p<0.01; p<0.01). Palmitic (C16:0), stearic (C18:0), oleic (C18:n-9), docosahexaenoic (C22:6n-3) and arachidonic (C20:4n-6) acid concentrations were mainly higher in the kidney and liver tissues which were collected from the T+PP and T+LU treated groups than those collected from the control group. The K1, delta-tocopherol and alfa–tocopherol acetate vitamin concentrations were higher in T+PP and T+LU treated groups than those at group treated with the toxin. Determined concentrations were similar to those measured in the control group. T+PP and T+LU decreased the cholesterol concentration in the kidney and liver tissues (p<0.05 and 0.01, respectively). According to results; while antioxidant defense systems of toluene groups have decreased, T+PP and T+LU possessed a protective feature against the oxidative stress caused by toluene. Key Words: Physalis peruviana, Linum usitatissimum, toluene, malondialdehyde, antioxidant, rat