Akademik Veri Yönetim Sistemi
Journal of the Hellenic Veterinary Medical Society, cilt.75, sa.2, ss.7611-7618, 2024 (SCI-Expanded)
Enteric syndromes, such as runting-stunting syndrome or malabsorption syndrome cause significant economic loss in industrial-scale poultry production. These syndromes are commonly thought to have a multifactorial aetiology; nevertheless, the extent of parvoviral involvement is still largely unknown. In addition, backyard flocks could be a potential reservoir for parvoviruses, in situations where fewer precautions are implemented. In this study, we aimed to investigate the potential contribution of chicken parvovirus to enteric diseases in farmyard layers owned by semi-professional producers by comparing different PCR primer sets. 404 faecal samples were collected from eight provinces during the period of January to May 2018. The presence of chicken parvovirus (ChPV) was investigated in all samples using five PCR methods, including nested and conventional PCR assays with different primer set combinations. Some samples were further sequenced, and the resulting sequences were analysed in silico. There was a significant variation in positivity percentages depending on the PCR method used (7.9% to 44.6%) in faecal samples from backyard flocks. Phylogenetic analysis further showed that the nucleotide identity of the Turkish strains ranged from 94.51% to 99.10 and that most of these strains fell into two distinct clusters. The nested PCR approach developed in this study could be an alternative to other conventional PCR primers due to its higher sensitivity. This is the first time that ChPV strains have been identified in Turkish poultry. overall, the results highlight the increasing health risk posed by ChPV infections in backyard poultry production and emphasize the need for biosecurity measures to prevent disease transmission.