Objective: The aim of this study was to prepare different nanoparticles using chitosan and tripolyphosphate and to perform characterization studies of nanoparticles. In addition, FTIR spectrum measurements were aimed to determine specific chemical groups and bonds in the structures of nanoparticles and materials. Method: In this study, different nanoparticles were prepared and their zeta potential, particle size and polydispersity index were measured. Moreover, specific chemical groups of nanoparticles and materials used in nanoparticles were determined by FTIR spectrum study. Morphological properties of nanoparticles were evaluated using scanning electron microscopy. Results: According to the zeta potential measurement results, A3 (18.66 ± 0.22) and A2 (14.32 ± 0.14) formulations showed the highest and the lowest zeta potential value, respectively. It was also observed that the A3 (284.22 ± 1.60) formulation had the lowest particle size. According to FTIR absorption spectrum results, a wide aliphatic O-H tension band of chitosan was observed between 1500 cm-1 -1100 cm-1. Conclusions: According to the results, it could be said that chitosan nanoparticles have the desired particle size, charge and morphological properties in terms of application. In FTIR spectrum results, bands showing specific chemical groups of chitosan, tripolyphosphate and A3 formulation were obtained.