The toxic effect of cypermethrin, amitraz and combinations of cypermethrin-amitraz in rats

KANBUR M., SİLİĞ Y., ERASLAN G., KARABACAK M., SOYER SARICA Z., Aahin S.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.23, sa.6, ss.5232-5242, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 6
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s11356-015-5720-z
  • Dergi Adı: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5232-5242
  • Anahtar Kelimeler: Toxicity, Cypermethrin, Amitraz, Combination, Rat, LIPID-PEROXIDATION, GLUTATHIONE-PEROXIDASE, BIOCHEMICAL PARAMETERS, INDUCED HEPATOTOXICITY, SUPEROXIDE-DISMUTASE, ANTIOXIDANT STATUS, CLINICAL-FEATURES, OXIDATIVE STRESS, PROTECTIVE ROLE, BREAST-CANCER
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this study, the effects of cypermethrin (CYP), amitraz (AMT) and combined cypermethrin-amitraz (CYP-AMT) on some serum biochemical, oxidative stress and drug-metabolising parameters were investigated in male Wistar albino rats. CYP, AMT and combined CYP-AMT were administered at doses of 80 mg kg(-1) bw(-1) of CYP and 170 mg kg(-1) bw(-1) of AMT for 1 day (single dose), and at doses of 12 mg kg(-1) bw(-1) of CYP and 25 mg kg(-1) bw(-1) of AMT for 40 days by oral gavage. Oxidative stress (malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G6PD)), serum biochemical (glucose, triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), creatinine, asparatate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein, albumin) in blood/tissues (liver, kidney, brain, spleen and testis) and hepatic drug-metabolising (cytochrome P450 2E1 (CYP2E1), NADH-cytochrome b(5) reductase (CYPb5), NADPH-cytochrome c reductase/NADPH cytocrome P450 reductase (CYTC), glutathione S-transferase (GST), glutathione (GSH)) parameters were measured in liver samples taken on days 1 and 40. In result, it was determined that CYP, AMT and their combinations led to significant changes in the parameters investigated, and it was ascertained that long-term exposure to insecticides and the administration of insecticide combinations produced greater toxic effects in comparison with the administration of insecticides alone.