Determination of VP2 sequence-based virulence motifs and phylogenetic analysis of domestic Turkish IPNV isolates

TAMER C., IŞIDAN H., Kalayci G., Ozan E., Ozkan B., ALBAYRAK H.

JOURNAL OF FISH DISEASES, cilt.45, sa.2, ss.327-334, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1111/jfd.13558
  • Dergi Adı: JOURNAL OF FISH DISEASES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.327-334
  • Anahtar Kelimeler: genotype, IPNV, Turkey, virulence, PANCREATIC NECROSIS VIRUS, TROUT ONCORHYNCHUS-MYKISS, FARMED RAINBOW-TROUT, AMINO-ACID-SEQUENCES, GENOME SEGMENT, 1ST REPORT, AQUABIRNAVIRUSES, IDENTIFICATION
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Infectious pancreatic necrosis (IPN) is a highly contagious disease of young salmonid fish and is one of the most severe economic diseases in aquaculture. In Turkey, an increase in infectious pancreatic necrosis virus (IPNV) outbreaks in freshwater rainbow trout have been reported in recent years. This study aimed to analyze the VP2 gene from recent IPNV isolates from Turkey to determine whether there are epidemiological links between IPNV isolates from rainbow trout (Oncorhynchus mykiss; 62) and sea bass (Dicentrarchus labrax; 1), wild turbot (Scophthalmus maximus; 1) and the environment in order to investigate potential wild and farmed fish interactions. In this study, 62 Turkish IPNV isolates collected over 10 years (2005-2014) from rainbow trout, sea bass and turbot were genotypically characterized. The phylogenetic analysis indicated that Turkish IPNV isolates are closely related to strains from Denmark, Iran and Spain and that all Turkish IPNV isolates belong to genogroup V, serotype A2 (Sp strain). Furthermore, low genetic diversity was found among the Turkish isolates (identity, 95.5%-100% nucleotides and 97.8%-100% amino acids). The result of the analysis of the amino acid residues found at positions 217, 221 and 247 (proline, threonine and alanine, respectively) could be associated with virulence.