Effect of captopril on the oxidative damage caused by pentylenetetrazole in the SHSY-5Y human neuroblastoma cell line

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Doğan M. , Karademir M.

Cumhuriyet Medical Journal, vol.42, no.4, pp.479-483, 2020 (International Refereed University Journal)

  • Publication Type: Article / Article
  • Volume: 42 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.7197/cmj.830835
  • Title of Journal : Cumhuriyet Medical Journal
  • Page Numbers: pp.479-483


Objective: Epilepsy is a crucial brain disorder that seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of captopril, on pentylenetetrazole (PTZ) induced epileptic seizures in SH-SY5Y cell line. 
Method: In this XTT cell viability assay, captopril was performed in vitro SH-SY5Y cell culture to evaluate PTZ-induced neurotoxicity. Tissue TOS concentrations at the cell supernatants were quantified with the automated assay method. Hydrogen peroxide was used for the calibration of the assay.
Results: When XTT cell viability results were evaluated, captopril did not affect neuronal viability in SH-SY5Y cell line. Moreover, captopril did not have significant effect on TOS levels (***P>0. 001)
Conclusions: Results showed that, captopril did not have neuroprotective properties in SH-SY5Y cell line after PTZ-induced neurotoxicity.