Akademik Veri Yönetim Sistemi
Turkish & Italian Joint International Animal Reproduction Congress, Antalya, Türkiye, 10 Ekim 2024, ss.253-256
Introduction and aim
The demand for assisting reproduction by artificial insemination in pet animals is increasing day by day and this application is becoming routine.
However, the fact that not all dogs give semen regularly and are not accustomed to this method is a disadvantage for the application. The aim of
this study was to use the method of short-term storage of semen from male dogs at +4°C to provide the opportunity to inseminate the female dog in the estrous cycle during the ovulation period with a single ejaculate. To achieve this, specific doses of nanoparticles will be added to the sperm extender to try to achieve the target (1–3).
Methods
In the study, sperm was collected from four fertile Kangal breed male dogs, aged 24-48 months, housed at the Sivas Cumhuriyet University Kangal Research and Breeding Center. Semen was obtained from the dogs by digital manipulation method. Semen was collected twice a week for a total of 4 times. In order to eliminate individual differences, the semen samples were pooled. Initially, sperm parameters were checked after each semen collection. Motility rate >80%, abnormal spermatozoon rate <20% and spermatozoon concentration >250x106 were determined. The sperm samples diluted with Tris-egg yolk extender were then divided into 7 equal parts (4). While one of the samples was kept for the control group, zinc nanoparticle 50 μg/ml (Z1), 100 μg/ml (Z2), 200 μg/ml (Z3) and activated carbon nanoparticle 50 μg/ml (C1), 100 μg/ml (C2), 200 μg/ml (C3) were added to the diluent respectively. Motility rates, live/dead rates, abnormal spermatozoon rates were checked every 8 hours starting from the 0th hour (5).
Results
When the short-term stored samples were evaluated, it was found that the total motility loss was the lowest in the groups in which 200 μg/ml activated carbon nanoparticle was added compared to the others (p<0.05). In addition, when zinc nanoparticle 50 μg/ml was added to the diluent, total motility loss was found to be less when compared with the control group (p<0.05). When the morphological examination results were evaluated, it was observed that there was no significant difference between the groups (p>0,05).
Discussion and conclusions
While direct references on nanoparticles added to dog semen extenders are limited, the existing literature on additives and nanoparticles in semen preservation provides a foundation for further exploration into the potential benefits of nanoparticles in dog semen extenders (6–11). As a result, it was determined that the addition of activated carbon and zinc as nanoparticles to the reconstituted semen obtained from kangal breed dogs showed longer motility, but the group showing sufficient motility was the group in which only 200 μg/ml activated carbon nanoparticles were added.