Purification and characterization of 1-dodecanol from an isolate of Streptomyces viridodiastaticus

ÇETİNKAYA S., YENİDÜNYA A. F., AKSU A., ÇELİK M. S.

Biocatalysis and Agricultural Biotechnology, cilt.34, 2021 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.bcab.2021.102013
  • Dergi Adı: Biocatalysis and Agricultural Biotechnology
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, BIOSIS, Food Science & Technology Abstracts
  • Anahtar Kelimeler: 1-Dodecanol, Characterisation, Electrophoresis, Purification, SDS-PAGE, Streptomyces viridodiastaticus, NANOPARTICLES, DNA, METABOLITES, EXTRACT
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

One-dodecanol was identified as the predominant secondary metabolite produced by a novel isolate of Streptomyces viridodiastaticus. A total secondary metabolite extract was electrophoresed, along with a prestained protein ladder (from 10 to 180 kDa), in a 10% SDS-polyacrylamide gel. Two prominent bands obtained were aligned with 25 and 10 kDa bands of the protein marker. These two secondary metabolite bands were also visualized by thin layer chromatography. The two bands, lower band (C1) and upper band (C2), in the polyacrylamide gel were sliced off and their metabolite content was eluted in n-butanol. Elution samples were analysed by GC-MS. Results indicated that the lower C1 band mostly contained 1-dodecanol, approximately 93%. The lower band also contained a 1-dodecanol-like metabolite, approximately 55%. The elution samples were also analysed by FTIR and the results were in accordance with those of the GC-MS.