Akademik Veri Yönetim Sistemi
3 rd International Hippocrates Congress on Medical and Health Sciences, Ankara, Türkiye, 6 Mart - 07 Eylül 2020, cilt.1, sa.1, ss.55-56
Aim: The first described vitamin B, thiamine (vitamin B1), acts as
cofactor for many enzymes in energy metabolism. Thiamine-bound
enzymes play important role in the synthesis of neurotransmitters, in
defense of oxidative stress and in cerebral metabolism. Nitric oxide
synthases (NOSs) are a family of enzymes catalyzing the production of
nitric oxide (NO) from l-arginine, and the NO is an important cellular
signaling molecule that modulates synaptic plasticity in the central nervous
system (CNS), central regulation of blood pressure, smooth muscle
relaxation, and vasodilatation via peripheral nitrergic nerves. The aim of
this study was to investigate the effect of thiamin on Nitric Oxide Synthase
in cortex and hippocampus after pentylenetetrazole (PTZ) - induced
epileptic seizures. Methods: In this study, 18 male Wistar Albino rats were
used. Animals divided into three groups as control, saline (serum
physiologic 1 ml/kg for 7 days) and thiamine (100 mg/kg for 7 days as
supplement). Seventh days after 30 min the administration of the last drug,
PTZ (45 mg/kg) was given to saline and thiamine groups to induced
seizures. After 24 hours of PTZ injection, brain tissues of all groups animal
were removed. Cortex and hippocampus separated and homogenized. NOS
was measured by using the sandwich-ELISA method in this homogenate.
Statistical evaluation of the data was performed by one-way ANOVA and
multiple comparisons were determined by the Tukey test. Statistical
significance was defined at p<0.05. Results: There is no statistical
significance on NOS levels in the cortex between groups (p>0.05).
However, there is increasing in NOS levels in the hippocampus for
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thiamine group compared to control and saline (p<0,05). Conclusion:
Thiamine increases NOS level in the hippocampus after seizures. It shows
that NOS regulation may play role in thiamin antiepileptic effect.
Anahtar Kelimeler: Thiamin, Epilepsy, Pentylenetetrazole, Nitric Oxide
Synthase, Rats