The Effect of Thiamin On Nitric Oxide Synthase in Cortex and Hippocampus After Pentylenetetrazole-Induced Epileptic Seizures

Şahin B.

3 rd International Hippocrates Congress on Medical and Health Sciences, Ankara, Turkey, 6 March - 07 September 2020, vol.1, no.1, pp.55-56

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.55-56


Aim: The first described vitamin B, thiamine (vitamin B1), acts as cofactor for many enzymes in energy metabolism. Thiamine-bound enzymes play important role in the synthesis of neurotransmitters, in defense of oxidative stress and in cerebral metabolism. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from l-arginine, and the NO is an important cellular signaling molecule that modulates synaptic plasticity in the central nervous system (CNS), central regulation of blood pressure, smooth muscle relaxation, and vasodilatation via peripheral nitrergic nerves. The aim of this study was to investigate the effect of thiamin on Nitric Oxide Synthase in cortex and hippocampus after pentylenetetrazole (PTZ) - induced epileptic seizures. Methods: In this study, 18 male Wistar Albino rats were used. Animals divided into three groups as control, saline (serum physiologic 1 ml/kg for 7 days) and thiamine (100 mg/kg for 7 days as supplement). Seventh days after 30 min the administration of the last drug, PTZ (45 mg/kg) was given to saline and thiamine groups to induced seizures. After 24 hours of PTZ injection, brain tissues of all groups animal were removed. Cortex and hippocampus separated and homogenized. NOS was measured by using the sandwich-ELISA method in this homogenate. Statistical evaluation of the data was performed by one-way ANOVA and multiple comparisons were determined by the Tukey test. Statistical significance was defined at p<0.05. Results: There is no statistical significance on NOS levels in the cortex between groups (p>0.05). However, there is increasing in NOS levels in the hippocampus for 56 thiamine group compared to control and saline (p<0,05). Conclusion: Thiamine increases NOS level in the hippocampus after seizures. It shows that NOS regulation may play role in thiamin antiepileptic effect. Anahtar Kelimeler: Thiamin, Epilepsy, Pentylenetetrazole, Nitric Oxide Synthase, Rats