Akademik Veri Yönetim Sistemi
Latin American International Congress on Natural and Applied Sciences-IV, Rio De Janeiro, Brezilya, 13 - 15 Mart 2023, ss.168-175, (Tam Metin Bildiri)
ABSTRACT
Inductively coupled plasma-mass spectroscopy, known as ICP-MS, is a spectroscopic device
that analyzes many elements in the dream table, such as trace elements, minerals and heavy
metals, which are basic inorganic things, with high selectivity and sensitivity. Materials
collected from living things such as humans, animals, plants, fungi, bacteria, and model
organisms constitute biological sample groups. These materials include; body fluids, tissue,
cells and many other components. Researchers need specific types of biological samples to
perform their work and advance medicine. The analysis of elements in biological samples is
very important in terms of clinical and routine analysis. Apart from this, studies that have
contributed to the literature in recent years have been carried out as a research and
examination subject. The reason why ICP-MS is highly preferred in biological samples is that
it has the ability to quickly measure many elements simultaneously, at very low detection
limits. Although ICP-MS is highly preferred in biological samples, various difficulties arise in
its use. These are low concentrations of the target species in a small amount of samples,
matrix effect, contamination and various interferences. The most common is the
contamination that may occur during sampling and storage. The materials used in the sample
collection stage should be carefully selected to avoid elemental contamination and should be
plastic. Plastic materials used in sample preparation and collection should be washed with 2%
nitric acid. Samples can be stored for a long time at +4-8 °C with pre-treatment, convert to
solution and acidification processes. Apart from this, in order to analyze biological samples
with ICP MS, it is necessary to convert the samples into liquid form and remove the majority
of their organic components. Biological samples cause matrix effect due to their organic
components and high salt content. Digestion of the organic part, dilution up to a certain rate,
is effective in reducing matrix interactions. In this sense, sample preparation is the first and
most important step in ICP-MS analysis. For this purpose, microwave combustion systems are
mostly used in sample preparation. Analyzing the sample by applying the sample directly to
the device in ICP-MS devices produced in different designs provides a great advantage in
biological samples. ICP-MS, which is highly preferred in routine clinical analyzes and
toxicology, is a powerful and analytical device with its high sensitivity and ability to quickly
analyze many elements at the same time. In recent years, research with the ICP-MS device
has gained momentum in many diseases and pathological conditions.