Investigation of leptospiral antigen with immunohistochemical and immunofluorescence methods in cattle kidney

SAĞLAM Y. S., YILDIRIM S., Ozkaraca M., ALTUN S.

Microbial Pathogenesis, cilt.164, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 164
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.micpath.2022.105434
  • Dergi Adı: Microbial Pathogenesis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Kidney, Immunohistochemistry, Immunofluorescence, Leptospira interrogans, Cattle
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this study, it was aimed to determine the presence of Leptospira interrogans antigens in the kidney samples of naturally infected cattle by immunohistochemical and immunofluorescence methods. 70 bovine kidney samples showing macroscopic lesions were examined by immunohistochemical and immunofluorescence methods. The positivity of Leptospira interrogans antigens was detected in 5 (7.14%) kidney samples examined by both methods. As a result; The presence of Leptospira interrogans antigens detected by IHC and IF staining methods in bovine kidneys where research samples were provided was found at the same rates (7.14%). Although it has low rates compared to previous studies, it has been determined that it is current and creates problems in terms of animal health.