Webinar on Life Sciences, Pennsylvania, United States Of America, 27 - 28 January 2021, pp.31
Giardia duodenalis is a unicellular flagellate parasite that infects the intestinal tract of a variety of
mammals, including humans.The life cycle of this protozoan is direct, cysts are eliminated in the
feces and are infective when ingested. Transmission occurs bythe fecal-oral route or by ingestion of
contaminated food and water. The aim of this study was to investigate the prevalence of G. duodenalis in
Anatolian Buffalos in the Kütahya region in Turkey. In this study A total of 80 Anatolian buffalos calves
fecal samples which were up to 6 months of age were collected some farms in Kütahya region in Turkey
during 2019-2020 and brought to the laboratory. After that, they were examined in terms of Giardia spp.
cysts under microscope by applying native examination method. Then DNA extraction was performed
according to the kit protocol by using QIAamp Fast DNA Stool Mini Kit. Nested PCR was performed with
appropriate primers. The obtained PCR products were run on 1.5% agarose gel stained with RedSafe™
Nucleic Acid Staining Solution and images were obtained using gel imaging device. Microscopically,
Giardia spp. cysts were found in 25 out of 80 (%31,25) examined samples. As a result of the Nested PCR,
30 (%37,5) samples were found positive in terms of Giardia duodenalis. It was determined that Giardia
duodenalis is quite commonly observed in Anatolian buffalos calves in the Kütahya region in Turkey.
Moreover, it was suggested that further precautions be taken.