In this study in-vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (alpha-glukosidase/alpha-amylase) and tyrosinase inhibitor activity of ethanol (80%) extract of different organs of Consolida regalis were determined. The stem extract showed stronger anticholinesterase (AChE and BChE) inhibition activity than the extracts from the other parts. While the strong alpha-glucosidase inhibition was determined in the leaf extract, the highest alpha-amylase inhibition activity has been shown in the stem extract. It was worth to pointing out that the ethanol extract of stem of the Consolida regalis demonstrated more tyrosinase inhibition activity than the reference compound and also showed higher -amylase inhibition activity than the antidiabetic drug-acarbose at the same concentration level. So these results may be helpful for development of novel pharmaceutical formulations to treat various diseases such as diabetes.