This study aimed to evaluate the effect of intracanal placement of various medicaments on the bond strength of ProRooT MTA, Biodentine, and Endosequence root repair material (ERRM) putty. Ninety extracted human mandibular premolar teeth were decoronated and instrumented using Protaper Next rotary system and #1 to #6 Peeso reamers. The prepared teeth were randomly divided into six groups according to the intracanal medicaments: Group 1: double antibiotic paste (DAP) consisting of metronidazole and ciprofloxacin; Group 2: Triple antibiotic paste (TAP) with cefaclor; Group 3: TAP with clindamycin; Group 4: amoxicillin and clavulanic acid (Augmentin (R)); Group 5: calcium hydroxide (CH); Group 6: control (no medicament) (n = 15). Parallel transverse sections were obtained in the coronal to the apical direction (4 slices/tooth) and were divided into three subgroups according to the cements: Group A: ProRoot MTA; Group B: Biodentine; Group C: ERRM (n = 20 slices/subgroup). A push-out test was used to measure bond strength. Data were analyzed using twoway analysis of variance and Tukey's post hoc test. The bond strength was significantly lower for DAP and TAPs than for Augmentin (R), CH, or the control (p < 0.05). Biodentine and ERRM had significantly higher bond strength values than ProRooT MTA (p < 0.05) regardless of the intracanal medicaments tested, while no significant difference existed between Biodentine and ERRM (p > 0.05). While the application of DAP or TAPs decreased the bond strength, application of CH or Augmentin (R) did not. ERRM and Biodentine had higher bond strength values than ProRoot MTA.