A new application of pH-dependent/charge transfer-sensitive spectrophotometer: Enhanced selective extraction of trace acrylamide in the selected foods by triplet quenching of Phenosafranine in presence of Sn(IV)

GÜRKAN R., Gürkan N.

Food Chemistry, cilt.508, 2026 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 508
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1016/j.foodchem.2026.148500
  • Dergi Adı: Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE
  • Anahtar Kelimeler: AAm, CT-sensitive spectrophotometry, Phenosafranine, Sn(IV), Thermally processed foods, Triton X-114, UA-CPE
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this study, a new method, based on pH-dependent charge transfer (CT), was developed for the selective extraction of acrylamide (AAm) from the sample matrix prior to its analysis. The method is based on CT-sensitive ion-pair formation among AAm, Phenosafranine (PSF), and Sn(IV) at pH 5.0, and extraction with polyethylene glycol tert -octylphenyl ether (Triton X-114). The ion-pair was enriched, diluted to 0.4 mL with methanol, and monitored at 640 nm by spectrophotometer. The variables affecting extraction efficiency were evaluated and optimized. Under optimal conditions, a recti-linear relationship, being 2.0-fold more sensitive below 10 μg L−1, was obtained in the range of 1–10, 10–180 μg L-1, with limits of detection and quantification (LODs and LOQs) of 0.20 and 0.67 μg L-1. From extraction of 25-mL sample solution, a sensitivity improvement and pre-concentration of 27.3 and 62.5-folds, respectively, were obtained. The recovery rate was in range of 95.2–103.6%, with a lower RSD than 5.5%. After the validation, the method was successfully applied to the analysis of the selected food samples.