Serum levels of amylase and lipase are the most widely used biochemical indicators of pancreatic inflammation. They are the basic markers used when making a diagnosis of pancreatitis. However, they are not necessarily the gold standard since the specificity and sensitivity of high amylase and lipase levels are low. The purpose of this study was to show the predictive value of C/EBP homologous protein in diagnosing acute pancreatitis. Thirty-two cases of acute pancreatitis that were admitted to the Department of Emergency Medicine of the Bezmialem Vakif University between November 2013 and July 2014 were analyzed. The diagnosis of acute pancreatitis was made based on characteristic signs and symptoms, increased serum enzyme levels, and abnormal findings on diagnostic imaging. When compared with the control group, the serum C/EBP homologous protein levels were found to be significantly increased with routine diagnostic methods in cases of acute pancreatitis. During the process of pancreatic inflammation, the level of C/EBP homologous protein increased independently in the etiopathogenesis of acute pancreatitis.