Anti-adhesive effect of naturally obtained dicalcium phosphate dihydrate nanoparticles in the rat uterine wound model Efecto antiadhesivo de nanopartículas de dihidrato de fosfato dicálcico obtenidas naturalmente en el modelo de herida uterina de rata

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Kurt B., Öksüz K. E., Sahin-Inan Z. D., Hepokur C.

Cirugia y Cirujanos (English Edition), cilt.91, sa.4, ss.457-467, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 91 Sayı: 4
  • Basım Tarihi: 2023
  • Doi Numarası: 10.24875/ciru.23000097
  • Dergi Adı: Cirugia y Cirujanos (English Edition)
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.457-467
  • Anahtar Kelimeler: Dicalcium phosphate dihydrate, Gynecologic surgery, Nanoparticles, Wistar rats
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Objective: In this study, we aimed to compare the anti-adhesive effects of contractubex and dicalcium phosphate dihydrate (DCPD) particles in rats treated with the uterine horn adhesion model. Materials and methods: Newly adult, 60 Wistar albino rats were used as experimental animals. The modified rat uterine horn adhesion model was used to induce intra-abdominal adhesion. Tumor necrosis factor (TNF)-α, interleukin (IL)-1, vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-β1 were studied for biochemical and immunohistochemical examination. Results: TNF-α decreased in each group, while it decreased more in G2 and G3 than in G1. IL-1β decreased in each group, while it decreased the most in G3. TGF-β1 and VEGF localization was less in the G2 compared to G1, the least TGF-β1 and VEGF immunolocalization was detected in the G3 and G4. For both antibodies, the least localization among all groups belonged to G3. From day 7 to day 21, the highest TGF-β1 immunolocalization was observed in G1, lesser localization in G2 and lowest in G3. Conclusion: DCPD nanoparticles show promise as a clinical antiadhesive agent and should be further evaluated in experimental animal models and human trials.