Determination of phenolic compounds in human saliva after oral administration of red wine by high performance liquid chromatography

Tartaglia A., Romasco T., D'Ovidio C., Rosato E., Ulusoy H. İ., Furton K. G., ...Daha Fazla

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.209, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 209
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.jpba.2021.114486
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Phenols, Red wine, FPSE, Green sample preparation, Human saliva, PHASE SORPTIVE EXTRACTION, WHOLE-BLOOD, QUANTIFICATION, PLASMA, BIOAVAILABILITY, SPECTROMETRY, RESIDUES
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Red wine is a relevant source of bioactive compounds, which contribute to its antioxidant activity and other beneficial advantages for human health. However, the bioavailability of phenols in humans is not well understood, and the inter-individual variability in the production of phenolic compounds has not been comprehensively assessed to date. The present work describes a new method for the extraction and analysis of phenolic compounds including gallic acid (Gal), vanillic acid (Van), caffeic acid (Caf), syringic acid (Sir); (-)-epicatechin (Epi); p-coumaric acid (Cum) and resveratrol (Rsv) in human saliva samples. The target analytes were extracted using Fabric Phase Sorptive Extraction (FPSE), and subsequently analysed by highperformance liquid chromatography (HPLC) coupled with photodiode array detector (PDA). Chromatographic separation was achieved using a Symmetry C18 RP column in gradient elution mode, with methanol and phosphate buffer as the mobile phases. The linearity (intercept, slope, and determination coefficient) was evaluated in the range from 1 to 50 mu g/mL. The limit of quantification (LOQ) was 1 mu g/mL (LLOQ >= 0.8 mu g/mL), whereas limit of detection was 0.25 mu g/mL. The intra and inter-day RSD% and BIAS% values were less than +/- 15%. The analytical performances were further tested on human saliva collected from healthy volunteers after administering red wine. To the best of our knowledge, this is the first FPSE procedure for the analysis of phenols in saliva, using a non-invasive and easy to perform sample collection protocol. The proposed fast and inexpensive approach can be deployed as a reliable tool to study other biological matrices to proliferate understanding of these compounds distribution in human body. (c) 2021 Elsevier B.V. All rights reserved.