Ydj1 but not Sis1 stabilizes Hsp70 protein under prolonged stress in vitro

Tutar L., Tutar Y.

BIOPOLYMERS, vol.89, no.3, pp.171-174, 2008 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 89 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1002/bip.20881
  • Title of Journal : BIOPOLYMERS
  • Page Numbers: pp.171-174


Yeast cytosol has two important co-chaperons; Ydj1 and Sis1. Genetic experiments showed that Ydj1 is not I essential for viability; however, cells lacking it grow very poorly at 30 degrees C or unable to grow at extreme temperatures. On the other hand, Sis1 is an essential protein and apparently plays a functional role at assembly or disassembly of protein complexes. Stability experiments revealed that only Ydj1-protected Hsp70 proteins can hydrolyze ATP under prolonged stress. (c) 2007 Wiley Periodicals, Inc.