MONITORIZATION OF PATULIN AND HYDROXYMETHYLFURFURAL IN FRUIT JUICES AND COMMERCIAL FRUITY BABY FOODS BY AN HPLC-DAD METHOD


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Dural E.

REVUE ROUMAINE DE CHIMIE, cilt.65, sa.2, ss.191-200, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Konu: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.33224/rrch.2020.65.2.08
  • Dergi Adı: REVUE ROUMAINE DE CHIMIE
  • Sayfa Sayıları: ss.191-200

Özet

Patulin as a mycotoxin and hydroxymethylfurfural as a chemicals decomposition product are the toxic compounds that are suspected to be cytotoxic and carcinogenic. The aim of this study was to develop a fast, convenient and reliable high-performance liquid chromatography (HPLC) method for the simultaneous analysis of patulin and hydroxymethylfurfural in 86 commercially available fruity baby foods (n=18) and fruit juices (n=68). Fruit juice or baby food sample (5 mL) was extracted with 10 mL ethyl acetate twice time and treated with 2% Na2CO3 solution. Afterwards, the extract was treated with 1 gr Na2SO4 and was filtered with Whatman (R) filter paper and the addition of 0.25 mL acetic acid onto obtained extract solution, it was filtered with 0.45 mu m disc filter and after the evaporation step, dissolution which was rebuilt with 500 mu L acidified water, applied to the HPLC as 20 mu L. The separation was carried out by the C-18 column (4.6x250 mm, 5 mu m i.d.) and quantitated with diode array detector (DAD) set at 276 nm. Recovery was >= 94.9%. Limit of quantification (LOQ) was 0.6 ng/mL for patulin and 0.3 mu g/mL for hydroxymethylfurfural HMF. The average level of patulin and hydroxymethylfurfural concentration were determined as 38.24 ng/mL +/- 39.64 and 7.24 mu g/mL +/- 7.68 in all samples. Also, unpermitted concentrations of patulin and hydroxymethylfurfural in the samples were detected as 27.9% and 16.7%, respectively. The detected concentration of PAT was higher in the fruit juices (p<0.001) than baby food samples.