IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters
BRAZILIAN ORAL RESEARCH, cilt.31, 2017 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31
- Basım Tarihi: 2017
- Doi Numarası: 10.1590/1807-3107bor-2017.vol31.0012
- Dergi Adı: BRAZILIAN ORAL RESEARCH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Anahtar Kelimeler: Interleukin-6, Interleukin-10, Polymorphism, Genetic, Aggressive Periodontitis, NECROSIS-FACTOR-ALPHA, GINGIVAL CREVICULAR FLUID, PROMOTER POLYMORPHISMS, INTERLEUKIN-6 GENE, ASSOCIATION, DISEASE, EXPRESSION, RISK, HAPLOTYPES, CYTOKINES
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sivas Cumhuriyet Üniversitesi Adresli: Evet
Özet
Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin (IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid (GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). GAgP patients showed significant improvement on clinical parameters after periodontal therapy (p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levels were observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6 (-174) and IL-10 (-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6 (-174) GG genotype had higher levels of GCF IL-10 levels.