THE PROPERTIES OF IMMOBILIZED INVERTASE ONTO A NEW SUPPORT MATERIAL; POLY(METHACRYLAMIDE/MALEIC ACID) COPOLYMERIC HYDROGEL


ÖZTOP H. N. , Catmaz F. B. , SARAYDIN D.

ACTA CHEMICA IASI, vol.26, no.2, pp.307-328, 2018 (Journal Indexed in ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.2478/achi-2018-0019
  • Title of Journal : ACTA CHEMICA IASI
  • Page Numbers: pp.307-328

Abstract

Poly (methacrylamide / maleic acid) PM/MA and poly (methacrylamide) PM hydrogels were prepared aiming to be used as a support for invertase. Spectrophotometric, thermal analysis methods, swelling and diffusion experiments were used for the characterization of hydrogels. The swelling of PM/MA was higher than that of PM in water. The diffusion of water within the hydrogel was found to be non-Fickian. Invertase was immobilized onto PM and PM/MA (samples named PM-I and PM/MA-I respectively). The optimum pH values were found to be; 6.0, 5.0 and 5.5 for free invertase, PM-I and PM/MA-I respectively. The optimum temperature values were found to be 30 degrees C, 35 degrees C and 40 degrees C for free invertase, PM-I and PM/MA-I respectively. The Michaelis constant (K-m) and maximum velocity of the enzymes (V-max) were K-m: 11,75 mM, V-max: 1,95 mu mol min(-1) for free invertase, K-m: 67,24 mM, V-max: 60,6 mu mol min(-1) for PM-I and K-m: 74,55 mM, V-max: 18,12 mu mol min(-1) for PM/MA-I. PM/MA-I showed excellent thermal, operational and storage stability.