This survey was carried out to determine some physico-chemical parameters and zooplankton structure in Karaman stream located in Southwestern Turkey. Some physico-chemical parameters and zooplankton structure in Karaman stream were assessed between 9 June 2007 and 9 December 2007. Water and zooplankton samples were collected from 4 different stations in Karaman stream. During the period of investigation 37 species of zooplanktons representing 3 groups namely Rotifera, Cladocera and Copepoda were determined in Karaman stream. Rotifera included 34 species, Cladocera 2 species and Copepoda 1 species were identified in Karaman stream. Of these, Macrochaetus subquadratus (Perty, 1850) belonging to Rotifera was a new record for Turkey. Rotifera were the most dominant group in the zooplankton of Karaman stream in this survey. In the water samples, totally 4 physico-chemical parameters were analyzed. As a result of this research, Karaman stream did not show any significant water pollution problem.