Tromethamine and dodecanol appear to be the major secondary metabolites of Streptomyces decoyicus M*

Çelik M. S., Aksu A., YENİDÜNYA A. F., ÇETİNKAYA S.

ARCHIVES OF MICROBIOLOGY, cilt.204, sa.8, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 204 Sayı: 8
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00203-022-03076-5
  • Dergi Adı: ARCHIVES OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: 1-Dodecanol, SDS-PAGE, Secondary metabolite, Streptomyces decoyicus, Tromethamine
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

An isolate of Streptomyces decoyicus M* (code of the isolate) was identified by the sequencing of 16S rRNA gene. It was grown on solid media and secondary metabolites were extracted with n-butanol. The extract was dried and run in a sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE, 10%). Two main bands obtained were sliced and the metabolites were regained in n-butanol. These two samples were then identified by gas-chromatography-mass spectrometry (GC-MS), and Fourier-transform infrared spectroscopy (FT-IR). The results demonstrated that tromethamine- and 1-dodecanol were the main constituents (band 1: 61% and 17.7%; band 2: 41% and 54%, respectively). This finding maintained that the isolate of Streptomyces decoyicus produced high amounts tromethamine- and 1-dodecanol under the conditions investigated.