Bromelain Protects Against PTZ-Induced Glial Damage and Inflammation: An In Vitro and In Silico Study

JOHA Z., Başgöz N., Özgür A., TAŞKIRAN A. Ş.

Cell Biochemistry and Biophysics, cilt.83, sa.3, ss.3215-3223, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 83 Sayı: 3
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s12013-025-01703-8
  • Dergi Adı: Cell Biochemistry and Biophysics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.3215-3223
  • Anahtar Kelimeler: Bromelain, Glial Cells, Inflammation, Neuroprotection, PTZ
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

This study aimed to investigate how bromelain protects glial cells from pentylenetetrazole (PTZ)-induced damage, focusing on its anti-inflammatory effects. C6 glioma cells were treated with PTZ, bromelain, or a combination of PTZ and bromelain. The interactions of bromelain with iNOS (Inducible Nitric Oxide Synthase) and COX2 (Cyclooxygenase-2) were investigated using molecular docking calculations. Cell viability was measured using the XTT (Methoxynitrosulfophenyl-Tetrazolium Carboxanilide) assay. iNOS, NO (Nitric Oxide), and COX2 levels were assessed using ELISA and immunofluorescence staining. Bromelain at 50 and 100 µg/mL significantly increased cell viability (p < 0.001). On the other hand, bromelain at 50 µg/mL reduced inflammation, as indicated by lower levels of NO, iNOS, and COX2 (p < 0.001). In-silico predictions suggest that bromelain can effectively target iNOS and COX2, key inflammatory proteins. These findings indicate that bromelain protects glial cells by exerting anti-inflammatory effects. However, further research is needed to understand the underlying mechanisms fully.