Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization
POLYMER-PLASTICS TECHNOLOGY AND MATERIALS, cilt.60, sa.1, ss.25-36, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 60 Sayı: 1
- Basım Tarihi: 2021
- Doi Numarası: 10.1080/25740881.2020.1784223
- Dergi Adı: POLYMER-PLASTICS TECHNOLOGY AND MATERIALS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.25-36
- Anahtar Kelimeler: In situ polymerization, invertase, invertase immobilization, nanocomposite, smart hydrogel, COMPOSITE HYDROGELS
- Sivas Cumhuriyet Üniversitesi Adresli: Evet
Özet
Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68 degrees C and 30.44 degrees C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH-I and NSH-I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH-I showed excellent thermal, operational and pH stability.