Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization

SARAYDIN D., ÖZTOP H. N., HEPOKUR C.

POLYMER-PLASTICS TECHNOLOGY AND MATERIALS, cilt.60, sa.1, ss.25-36, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/25740881.2020.1784223
  • Dergi Adı: POLYMER-PLASTICS TECHNOLOGY AND MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.25-36
  • Anahtar Kelimeler: In situ polymerization, invertase, invertase immobilization, nanocomposite, smart hydrogel, COMPOSITE HYDROGELS
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68 degrees C and 30.44 degrees C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH-I and NSH-I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH-I showed excellent thermal, operational and pH stability.