Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68 degrees C and 30.44 degrees C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH-I and NSH-I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH-I showed excellent thermal, operational and pH stability.