Akademik Veri Yönetim Sistemi
Veterinary Sciences and Practices, cilt.18, sa.2, ss.47-51, 2023 (Scopus)
Bovine viral diarrhea virus (BVDV) is an important pathogen that causes diseases in the gastro-intestinal, respiratory, and reproductive systems. It also leads to a decrease in reproductive per-formance and consequently continuous economic losses in cattle management. The presence of BVD virus in bull testes was investigated in this study using indirect immunofluorescence (IF), immunohistochemistry (IHC), and Reverse Transcription polymerase chain reaction (RT-PCR) methods. The tissue distribution of BVDV was examined pathologically and virologically in 100 bull testis tissue samples. For this purpose, IF, IHC, and RT-PCR methods were employed. Positive IF staining was detected in 21 (21%) testis samples using the indirect IF method. In IHC staining, 16 (16%) samples were found to be positive. In RT-PCR, 13 (13%) samples tested positive. The results of the presented study demonstrate that BVDV can infect different cell types in bull testes and that the virus can primarily be transmitted through natural or artificial insemination. Therefore, bulls are an important epidemiological factor in the transmission of the disease.