Removal of safranin-O dye from wastewater samples using Kluyveromyces marxianus biomass: Experimental research and theoretical analysis


Akin M. B., Sari M., Eyupoglu V., Çetintaş H. I., Tüzün B., Çetinkaya S.

Journal of Molecular Liquids, cilt.436, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 436
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.molliq.2025.128198
  • Dergi Adı: Journal of Molecular Liquids
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Anahtar Kelimeler: Biosorption, DFT, Kluyveromyces marxianus, Molecular docking, Safranin-O
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Safranin-O was adsorbed onto the dead mass of Kluyveromyces marxianus in aqueous milieu and its outcome was assessed by analytical, visual, and computational approaches. The biosorbent was isolated from a kefir sample and the biomass of the biosorbent was used in the removal study. Dye-binding active moieties on the surface were identified by FT-IR (Fourier Transform Infrared Spectrophotometry). Topology images before and after adsorption were produced by SEM (Scanning Electron Microscope) and EDX (Energy Dispersive X-Ray Analysis). Critical reaction parameters, pH, PZC, adsorbent mass, binding time- and temperature were adjusted. Modeling of the adsorption process and recovery experiments were carried out. The best correlation coefficient (R2) was produced by Langmuir and Temkin models. Nonlinear models provided an overall better fit with the experimental data. The application of pseudo first order (PFO), pseudo second order (PSO), and intraparticle diffusion (IPD) models helped elucidate kinetics of the reaction. Recovery experiments were conducted thrice. Molecular docking was used to study the nature of the interaction between safranin O and the biosorbent, to better understand such interactions and to determine how effectively the biosorbents bind safranin O.