Biological effects and molecular docking studies of Catechin 5-O-gallate: antioxidant, anticholinergics, antiepileptic and antidiabetic potentials


Taslimi P., KOÇYİĞİT Ü. M. , TÜZÜN B. , KIRICI M.

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2020 (SCI İndekslerine Giren Dergi) identifier identifier identifier

Özet

Gallocatechin gallate is a form of catechin and an ester of gallocatechin and gallic acid. This is an epimer of the gallate epigallocatechin. In this study, the effect of this molecule, containing a biologically active group, was investigated in terms of important metabolic enzymes (carbonic anhydrase isoenzymes I and II (hCA I and II), achethylcholinesterase (AChE) and alpha-glycosidase (alpha-Gly) enzymes). The molecular docking method used to compare the biological activities of the Catechin 5-O-gallate molecule against enzymes was used. Afterwards, the ADME/T analysis was performed to investigate the drug availability of the Catechin 5-O-gallate molecule and the parameters obtained from ADME/T analysis were examined. Continuation of this study, for evaluating antioxidant and radical scavenging capacity Catechin 5-O-gallate, cupric ion (Cu2+) reduction capacity by CUPRAC method, Fe3+-Fe2+ reducing capacity, DPPH free radical clarifying (DPPHGreek ano teleia), ABTS radical clarifying (ABTS(center dot+)) were performed separately and during the study, trolox, alpha-tocopherol BHT and BHA were used as the reference antioxidant compound. Comparisons were applied with the four standard substances.