Akademik Veri Yönetim Sistemi
PAKISTAN VETERINARY JOURNAL, cilt.42, sa.2, ss.322-329, 2024 (SCI-Expanded)
Bovine respiratory disease complex (BRDC) is one of the most common cattle
diseases, causing widespread effect in worldwide. That pathogens causing BRDC
occur with coinfections may even lead irreversible losses. That is why, in this study,
it is aimed to investigate crucial viral pathogens associated with BRDC [Bovine
herpesvirus-1 (BoHV-1); bovine respiratory syncytial virus (BRSV), bovine viral
diarrhoea virus (BVDV), Hobi-like pestivirus, bovine parainfluenza-3 virus (BPIV
3)] in the same sample simultaneously. Aligned with the aim, 129 nasal swabs were
collected from cattle with clinical symptoms of respiratory system disease in specific
provinces of eastern and southeastern Türkiye. Firstly, viral RNA and DNA were
isolated from the samples. Subsequently, PCR and RT-PCR analyses were conducted
using specific primers for the viral pathogens. The results illustrated 10.85 BRSV,
1.55 BVDV-1 and 3.8% BPIV-3 positivity rates. BoHV-1, Hobi-like pestivirus, and
mixed infection were not detected in any of the samples. Samples that tested positive
were sequenced to represent each province for molecular characterisation and
phylogenetic analysis. They were determined that the BRSV strains (TR/Bngol
04/BRSV, TR/Dyrbkr-17/BRSV, and TR/Elz-23/BRSV) obtained from this study are
a part of subgroup III, BVDV strains (BVDV-1/TR-/Malatya-1 and BVDV
1/TR/Elazig-29) belonged to subgroup BVDV 1l, and the BPIV-3 strains
(BPIV3/M17/TUR and BPIV3/M19/TUR) were within genotype C. The findings of
these studies can be valuable to minimise the losses attributed to BRDC by
facilitating the development of diagnostic, molecular, and epidemiological
approaches. These insights can also serve as a foundation for establishing targets for
vaccine development, ultimately contributing to the reduction of BRDC.