Investigation of in Vitro Activity of the Novel Cationic Steroid Antibiotic CSA-13 against Toxoplasma gondii

Ataş A. D., Akın Polat Z., Çetin A., Savage P.

Baltica, cilt.34, sa.10, ss.1-10, 2021 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 10
  • Basım Tarihi: 2021
  • Dergi Adı: Baltica
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Central & Eastern European Academic Source (CEEAS), Environment Index
  • Sayfa Sayıları: ss.1-10
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Background: The aim of this study is to investigate the in vitro evaluation of cells (L929 mouse fibroblast cell) infected with Toxoplasma gondii tachyzoites and intracellular replication in the presence of CSA-13 from cationic steroid antibiotics. Methods: This study was conducted at the Parasitology Laboratory of Medical Faculty of Sivas Cumhuriyet University, Sivas, Turkey. CSA-13 cholic acid was synthesized using the "scaffold-configuration technique”. The effects of CSA-13 cholic acid on T. gondii tachyzoites were investigated in cell culture medium. Cytotoxic potentials of 200, 100, 50, 25, 12.5, 6.25, 3.12, 1.6 and 0.8 μM concentrations of CSA-13 and Trimethoprim (TMP) used as controls were determined by the WST-1 method. The cells were infected with T. gondii (RH) tachyzoites and treated starting from the highest non-cytotoxic dose of CSA-13, and viable cell numbers at 24, 48, 72 and 96 hours, was also detected by the WST-1 method. The results were evaluated statistically. Results: It was determined that low concentrations of CSA-13 starting at 6.25 μM were not cytotoxic and anti-proliferative evaluation was initiated at this concentration. All doses of trimethoprim used in the experiment were found to be non-cytotoxic. CSA-13 generally inhibited the proliferation and growth of T. gondii-infected cells when compared to TMP. Conclusion: This in vitro study shows that CSA-13 has potency to be used as a promising anti-T. gondii candidate substance for the development of effective antiparasitic drugs, particularly in the acute phase of T. gondii infection. Keywords: Cationic steroid antibiotic, CSA-13, in vitro activity, tachyzoites, Toxoplasma gondii.