Akademik Veri Yönetim Sistemi
Diagnostic microbiology and infectious disease, cilt.115, sa.3, ss.117369, 2026 (SCI-Expanded, Scopus)
contribution of aerobic and anaerobic culture bottles in Brucella spp. detection using automated blood culture
systems.
Methods: This retrospective study included 95 patients diagnosed with brucellosis. A total of 175 blood culture
bottles were analyzed, consisting of 171 aerobic and 4 anaerobic bottles. Time to detection (signal time) for
Brucella spp. was recorded. Hematological and biochemical parameters were compared between patients with
and without focal organ involvement.
Results: The median signal time for Brucella spp. detection was 62.2 h in aerobic cultures and 146.8 h in
anaerobic cultures, a statistically significant difference (p = 0.007). All positive aerobic cultures signaled within
the first 7 days. Diabetes Mellitus (DM) were associated with shorter signal times. Patients with focal organ
involvement had significantly higher white blood cell counts, neutrophil counts, and blood urea nitrogen (BUN)
levels compared to those without focal involvement. However, the laboratory findings associated with this
involvement were not correlated with signal times.
Conclusions: Automated blood culture systems reliably detect Brucella spp. within 7 days, rendering extended
incubation unnecessary. Anaerobic cultures showed minimal diagnostic value and may be omitted in suspected
brucellosis cases. Moreover, the presence of DM was observed to shorten the signal time; however, the literature
lacks studies that have comprehensively examined the relationship between DM and poor prognosis in brucellosis.
These findings highlight the need for further research on the potential association between signal time and
relapse, as well as the impact of DM on brucellosis outcomes.