2,4-dinitrophenol (DNP) was covalently bonded to glassy carbon substrate by cyclic voltammetry (CV) technique. 2, 4-diaminophenol film (DAPO) was acquired with electrochemical reduction of 2,4-dinitrophenol film (DNPO). Prepared films were characterized by X-ray photoelectron spectroscopy (XPS) and germanium attenuated total reflection infrared (GATR-FT-IR) spectroscopy. A hemispherical germanium ATR element used with p-polarized light at 65 incidence angle yielded high signal/noise IR spectra for monolayer coverage of molecules on glassy carbon substrate. Layer thickness of films was measured using ellipsometer. Nitro and amine groups involved surface were commented on mono-multi layer film thickness with modified cyclic number effects. (C) 2013 Elsevier B.V. All rights reserved.