A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.
Acta virologica, cilt.44, sa.1, ss.29-33, 2000 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 44 Sayı: 1
- Basım Tarihi: 2000
- Dergi Adı: Acta virologica
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.29-33
- Sivas Cumhuriyet Üniversitesi Adresli: Evet
Özet
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned sequenced expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.